Dulbecco’s Modified Eagle’s Medium/ Nutrient Ham’s Mixture (DMEM/F-12)

Dulbecco’s Modified Eagle’s Medium/Nutrient Ham’s Mixture (DMEM/F-12) is a 1:1 mixture of DMEM and Ham’s F-12 medium, containing DMEM’s high concentrations of glucose, amino acids, and vitamins with F-12’s wide variety of components, without proteins, lipids, or growth factors.

Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. This product is a GMP grade medium without animal-derived components. DMEM/F-12 requires additional culture additives such as fetal bovine serum. It uses a sodium bicarbonate buffer system and therefore requires a 5% ~ 10% CO2 environment to maintain physiological pH.

Requires additional additives
Glucose, 3151 mg/L
With L-glutamine
Without antibiotics
With sodium pyruvate
With sodium bicarbonate
With phenol red
Without HEPES