| Product Type |
WB |
| Product Summary |
- Fast Western Blot Transfer Buffer 10× is a transfer buffer used in Western blot. This buffer generates low heat, has a fast transfer rate, and has a good transfer effect on macromolecular proteins that are difficult to transfer. At the same time, this buffer can shorten the time difference between small molecule proteins and large molecule proteins for membrane transfer, and achieve efficient transfer of proteins of different molecular weights on the same membrane.
|
| Product Component |
| Product Name |
Cat No. |
Size |
| Fast Western Blot Transfer Buffer 10× |
8015071 |
500 mL |
| 8015072 |
5 L |
|
| Usage |
- According to the buffer volume required in the transfer tank, prepare 1× Transfer Buffer in the ratio of Transfer Buffer (10×): methanol/ethanol: water = 1:2:7, mix well and set aside.
- Prepare the equipment, transfer membrane and gel required for transfer.
- Add 1× Fast Western Blot Transfer Buffer to the transfer tank, set the electrophoresis apparatus to a constant current of 400 mA (voltage is approximately in the range of 75 V~85 V), and start transfer.
|
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