Four-color Fluorescence kit
$1,014.00 – $3,899.00
Product will arrive in 2-3 weeks
Overview
√ .Multiple targets can be labeled simultaneously.
√ .Flexible choice of primary and secondary antibodies.
√ .Strong signal, resistant to fluorescence quenching.
√ .Panoramic detection of protein expression, rich in information.
Size
20 Tests/50 Tests/100 Tests
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Product Details
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Data Images
Citations
Product Documents
Product Details
| Product Type | Four-color Fluorescence kit |
| Product Summary | Primary antibody forms antigen-antibody complex with target antigen in the section, the reagent after primary antibody in this kit closes the non-specific protein and improves the binding efficiency, the ultrasensitive enzyme-labeled goat anti-mouse/rabbit IgG polymer binds to the primary antibody in the antigen-antibody complex, the fluorescent diluent diluted TSA fluorescent reagent can be catalyzed by the ultrasensitive enzyme-labeled goat anti-mouse/rabbit IgG polymer of the HRP to produce an activated fluorescent substrate, the activated substrate can covalently bind to residues such as tyrosine on the antigen to make stable covalent binding TSA fluorescent reagent on the samples. The substrate can covalently bind to tyrosine and other residues on the antigen, resulting in stable covalent binding of TSA fluorescein on the sample. Afterwards, the non-covalently bound primary antibody-secondary antibody-HRP complex is washed away by thermal repair, and the next primary antibody-secondary antibody-HRP is repeated for the second round of incubation, replaced by another TSA fluorescent reagent, and so on to achieve multiple labeling, fluorescent DAPI reagent re-staining to locate the nucleus, and anti-fluorescent quenching sealing reagent is used to seal the film. |
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| Product Component | 20 Tests
50 Tests
100 Tests
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Storage & Preparation
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Data Images
Pic 1: Multiplex immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using CD4 & TSA 488, Ki-67 & TSA 546, CD8 & TSA 647 and DAPI. False colors have been specified, as shown in the image;
Citations
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Contact Us |
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