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Overview
√ Freezing lymphocyte samples are often used as test cells in the application of ELISPOT assay. Since the ELISPOT assay targets at immune function of cells, it is not only necessary to ensure the cell viability, but also to ensure that the cell function remains unchanged.
√ Considering the particularity of ELISPOT assay, Dakewe launched BioSci™ Freezing Kit, supplemented with a series of protectants with special protective effects on cells to the freezing medium.
√ Dakewe did experiments on cell freezing and thawing in its self-build laboratory and several Chinese clients’ laboratories, and performed ELISPOT assays for the thawed cells. The results showed that the viability of lymphocytes was more than 90%, and the frequency of ELISPOT spot formation was exactly the same as that of fresh cells.
Size
25mL + 25mL /1 kit
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Product Details
Storage & Preparation
Data Images
Citations
Product Documents
Product Details
Product Type | Cell Freezing reagents |
Usage | This product is used to freeze lymphocyte samples for ELISPOT assay.Since the ELISPOT assay targets at immune function of cells, it is not only necessary to ensure the cell viability, but also to ensure that the cell function remains unchanged. |
Product Components | 25 mL Resuspension medium
25 mL Freezing mdium |
Note | Avoid repeated freezing and thawing of this product, and pay attention to operate aseptically during use. |
Storage & Preparation
Shipping | Shipped with Dry Ice. |
Stability & Storage | Store at -15℃ ~ -25℃ before use and at 2℃ ~8℃ after thawing. This product can be stored at 2℃ ~8 ℃ for at least 6 months. |
Data Images

Frozen and thawed human PBMCs can undergo ELISPOT assay directly without the need to rest overnight. All PBMCs should be incubated directly in human IFN-γ ELISPOT kit containing serum-free medium for 18 h.
Citations
Product Documents
Product Datasheet | Download Product Datasheet |
COA | Contact Us |
SDS | Download SDS |
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