Gel/PCR DNA Purification Kit
$44.37
Price Not Effetive Due to Tariff After April 10
- Gel/PCR DNA Purification Kit uses a unique adsorption column to both recover DNA fragments from agarose gels and directly purify PCR products. The Binding Buffer of the kit contains a pH indicator, which can judge the binding efficiency of DNA to the membrane by the color of the solution and improve the recovery rate of DNA.
- The kit has the characteristics of high efficiency and fast, which can recover 65 bp~10 kb DNA fragments, the gel recovery rate can reach 70%~85%, and the purification efficiency of PCR products can reach 90%~95%. A single adsorption column can adsorb 20 μg DNA per time, and the operation can be completed in 10~20 minutes.
- The DNA recovered from this kit can be used for a variety of routine operations, including enzyme digestion, PCR, sequencing, library screening, ligation and transformation experiments.
Product will arrive in 2-3 weeks
Gel/PCR DNA Purification Kit uses a unique adsorption column to both recover DNA fragments from agarose gels and directly purify PCR products. The Binding Buffer of the kit contains a pH indicator, which can judge the binding efficiency of DNA to the membrane by the color of the solution and improve the recovery rate of DNA.
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| Product Type | DNA Extraction and Assay Kit | ||||||||||||||||||||||||||
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| Usage | Gel extraction scheme: used to recover DNA fragments from agarose gel. 1. Use a clean scalpel to cut the target DNA strip from the agarose gel and put it into a 1.5 mL centrifuge tube. 2. Add 500 μL Binding Buffer of up and down to mix well. 3. Incubate at 55℃ for 5~10 minutes, turn up and down every 2~3 minutes until the gel is completely dissolved, and then conduct the next operation after cooling to room temperature. 4. Place the Spin Column into the Collection Tube. Mix the sample solution (≤ 800 μL) Transfer to Spin Column, centrifuge 11000×g for 30 seconds, and discard the filtrate. 5. Add 750 μL Wash Buffer to Spin Column, centrifuge 11000×g for 30 seconds, and discard the filtrate. 6. Put Spin Column back into the Collection Tube, centrifuge 18000×g for 3 minutes, and thoroughly remove residual Wash Buffer. 7. Place Spin Column in a new 1.5 mL centrifuge tube, add 20 μL~40 μL of Elution Buffer or ddH2O to the center of Spin Column membrane, and stand for 1 minute. Centrifuge at 18,000×g for 1 minute to elution DNA, and store at -20°C. PCR Purification Protocol: For Purification of PCR Products or Reaction Mixtures. 1. Transfer the PCR product (≤100 μL, without mineral oil) into a new 1.5 mL centrifuge tube, add 5 times the volume of Binding Buffer, and vortex to mix. 2. Put the Spin Column into the Collection Tube. Transfer the sample mixture into the Spin Column, centrifuge at 11,000×g for 30 seconds, and discard the filtrate. 3. Add 750 μL Wash Buffer into the Spin Column, centrifuge at 11,000×g for 30 seconds, and discard the filtrate. 4. Put the Spin Column back into the Collection Tube, and centrifuge at 18,000×g for 3 minutes to completely remove the residual Wash Buffer. 5. Place the Spin Column into a new 1.5 mL centrifuge tube, add 20 μL~40 μL Elution Buffer or ddH2O to the center of the Spin Column membrane, and let stand for 1 minute. Centrifuge at 18,000×g for 1 minute to elution DNA, and store at -20°C. |
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| Notes | a. Add 100 mL ethanol (96%~100%) to Wash Buffer before the first use. b. The gel should be cut quickly under UV to avoid DNA damage caused by long time UV irradiation. c. The centrifuge operation can be carried out at room temperature of 11,000×g~18,000×g. d. If DNA is used for sequencing, it is recommended to use ddH2O elution. Elution Buffer was recommended if DNA was stored for a long time. e. This product is limited to the scientific research of professionals. For your safety and health, please wear a laboratory coat and wear disposable gloves to operate. |
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| SDS | Download SDS |
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