Mag Beads Plasmid Extraction Kit
$76.06
Price Not Effetive Due to Tariff After April 10
- DNA binds to the surface of silicon-coated Magbeads under high salt conditions. After multiple washes to remove impurities such as proteins, DNA is eluted under low salt conditions, resulting in high-purity DNA.
Product will arrive in 2-3 weeks
Overview
- Efficiency and speed.
- High purity and recovery.
- Automate and streamline operations.
- Environmental protection and safety.
- Compatibility and low cross-contamination risk.
Need Help Ordering?
Product Details
Storage & Preparation
Data Images
Citations
Product Documents
Product Details
| Product Type | Nucleic Acid Extraction | ||||||||||||||||||||||||||||||||||
| Product Summary |
|
||||||||||||||||||||||||||||||||||
| Product Component |
|
||||||||||||||||||||||||||||||||||
| Usage | 1. Take 2~5 mL of bacterial suspension into a suitable centrifuge tube, centrifuge at 12,000 g for 2 minutes, and discard the supernatant. Fully suspend the bacterial precipitate in 300 μL Buffer 1 (confirm if RNase A is added). 2. Add 300 μL Buffer 2 and gently invert 5~6 times to fully lyse the bacteria and form a clear solution. 3. Add 150 μL Buffer 3, gently invert 6~8 times to form a compact flocculent mass. Centrifuge at 12,000 g for 10 minutes at room temperature, transfer 600~700 μL supernatant to a new centrifuge tube, add 20 μL magnetic beads, and 400 μL isopropanol. Shake at room temperature for 5 minutes to combine. 4. Place the centrifuge tube on the magnetic rack and let it stand for 30 seconds until the solution clarifies, then remove the supernatant as much as possible. 5. Add 500 μL wash solution, vortex for 30 seconds, resuspend the magnetic beads, then place the centrifuge tube on the magnetic rack for 30 seconds until the solution clarifies, and remove the supernatant. 6. Add 500 μL 80% ethanol, vortex for 20 seconds, resuspend the magnetic beads, then place the centrifuge tube on the magnetic rack for 30 seconds until the solution clarifies, remove the supernatant, and remove residual droplets from the tube wall as much as possible. 7. Repeat step 6 once. 8. Ventilate in a fume hood for 5 minutes (ensure ethanol is completely evaporated). 9. Add 50~100 μL elution buffer to the magnetic beads, vortex thoroughly, and shake at room temperature for 5 minutes (for better results, shake at 55°C constant temperature for 5 minutes). 10. Note: Before adding the elution buffer, it can be heated in a water bath to 55°C to facilitate better elution of plasmid DNA. 11. Place the centrifuge tube on the magnetic rack. After magnetic absorption is complete and the solution is clear, aspirate the supernatant and transfer it to a clean centrifuge tube for storage at -20°C。 Others: See the Datasheet for details of Manual Operation Steps-96-Well Plate Operation and Automated Operation Steps for 96-Channel Nucleic Acid Extractor. |
||||||||||||||||||||||||||||||||||
| Notes |
|
Storage & Preparation
| Storage |
|
| Transportation |
|
Data Images
Citations
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Download SDS |
Reviews
There are no reviews yet.