Mouse Lymphocyte Separation Medium

Characteristics

• This product is with high purity, good state and high yield.
• The operation of Mouse Lymphocyte Separation Medium is simple, easy to learn and does not need too much experience.

• Sacrifice the mouse by cervical dislocation; dip it in 75% ethanol.
• Take out the spleen of the mouse at a clean bench. Operation shall be performed in an aseptic condition.
• Add 4~5 mL Mouse Lymphocyte Separation Medium (pre-warming at room temperature and shaking before use) into a 35 mm petri dish. And grind (please refer to Figure 1 for grinding operation). The nylon mesh or cellular screen containing the spleen is dipped into the separationsolution and the spleen is ground in the separation solution. Refer to Figure 1 for grinding operation.

• Iodixanol (chemical inertness, no biological toxicity), 100 mL

• Ambient Temperature.

• The product shall be stored at 4 °C -30 °C and protected from light. Its shelf life is 2 years.