NewFlash Protein PAGE For High MW

• NewFlash Protein PAGE For High MW is a product with a low acrylamide concentration, and adopts the latest gel technology to reduce the concentration of acrylamide and enhance the gelation rate. The kit is easy to use, and prepares gel quickly and safely.
• Protein gel preparation is simple, and can be completed in 25 min by mixing resolving gels and stacking gels at the same time.
• Protein gel electrophoresis can be completed either at 300 V constant high voltage in about 25 min or at normal voltage.
• The kit is suitable for separation and identification of 50-300 kDa protein, and for separation and identification of high molecular weight proteins.

1. With Biosci™ NewFlash Protein PAGE For High MWkit, uniformly mix high molecular weight resolving gel solutions A and B at a ratio of 1:1 (As a reference, for a 0.75/1.0/1.5 mm gel, respectively draw 2.0/2.5/3.8 mL of high molecular weight resolving gel solutions A and B). Gel preparation can be completed in a 15 mL or 50 mL centrifuge
2. With Biosci™ NewFlash Protein PAGE For High MWkit, uniformly mix high molecular weight stacking gel solutions A and B at a ratio of 1:1 (As a reference, for a 0.75/1.0/1.5 mm gel, respectively draw 0.8/1.0/1.5 mL of high molecular weight stacking gel solutions A and B).

Note: With this product, gelation is rapid. Do not wait for pouring the resolving gel into the glass plate to start the preparation of stacking gel. Excessive waiting time will lead to partial polymerization of the resolving gel, resulting in uneven division surface of resolving gel and stacking gel, which will influence the electrophoresis effect.

3. Add 10% APS solution to the high molecular weightresolving gel solution in step 1 (for 5 mL of high molecular weight resolving gel solution, add 50 µL of 10% APS solution), and pour the uniformly mixed resolving gel solution into the glass plate to a position 1.5 cm from the top of the front glass plate or 0.5 cm from teeth of a comb.

Note: When it is necessary to prepare more than one protein gels at one time, the amount of APS can be appropriately reduced to lower the gelation rate. Excessive APS will result in gel embrittlement.

4. Add 10% APS solution to the high molecular weightstacking gel solution in step 2 (for 2 mL of high molecular weight stacking gel solution, add 20 µL of 10% APS solution), and directly pour the uniformly mixed stacking gel solution on the resolving gel solution without waiting for solidification of the resolving gel solution.

Note: The kit can complete protein gel casting at one time without mixing stacking gel with resolving gel.

5. Insert the comb into the gel, and keep it standing for 15-20 min to wait for gel polymerization.

Note: Gels can be stored for several weeks at 2~8℃ in sealing bags with a small amount of electrophoresis buffer.

6. After gel polymerization, remove the comb, and purge gel pores with a 1 mL injector or a pipette for sample loading. It is recommended that high molecular weightprotein marker shall be used to indicate the molecular weight of the protein samples on PAGE gel.
7. The electrophoresis of this product can be carried out rapidly in a conventional electrophoresis buffer. The voltage can be set to a maximum of 300 V, at which electrophoresis can be completed in about 25 min. The current shall not exceed 140 mA. The voltage can be reduced if the current is too high (to avoid much heat).

Note: The electrophoresis of this product can be carried out at normal voltage.

• Stored at 2℃~8℃ protected from light, with a shelf life of 24 months.

• Transported on blue ice.