Nicking Enzymes Nt.BstNBIⅠ
$240.85
Price Not Effetive Due to Tariff After April 10
- Nicking Enzymes Nt.BstNBⅠ is a nicking enzyme which digests only one strand of dsDNA substrate. The digested dsDNA substrate would produce a nick and not be completely digested. It is suitable for nicking enzyme amplification reaction, preparation of probe, assembly of large DNA fragments, etc.
Product will arrive in 2-3 weeks
Overview
✓ Nicking enzyme amplification reaction
✓ Preparation of probe
✓ Assembly of large DNA fragments
✓ Digest only one strand of dsDNA substrate
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Product Details
| Product Type | Nicking Enzymes | ||||||||||||
| Product Summary | Nicking Enzymes Nt.BstNBⅠ is modified as follows:
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| Notes | a. The DNA substrate should not contain phenol, chloroform, ethanol, EDTA, detergent or high-concentration salt, otherwise it will affect the activity of Nicking Enzymes Nt.BstNBⅠ enzyme. b. The combined volume of the enzymes in the reaction mixture should not exceed 1/10 of the total reaction volume. c. The additives (such as glycerol, salt) in the enzyme storage buffer are the same as the contaminants (such as salt, EDTA, ethanol, etc.) in the substrate solution. Therefore, the smaller the reaction volume, the stronger the inhibitory effect of the enzyme cleavage reaction. |
Storage & Preparation
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Data Images
Citations
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Download SDS |
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