Prestained Agarose Gel Electrophoresis Kit

Usage1. Preparation:
a. Configure 1× TAE buffer as figure 1.

b. Take out one kit, take off the plastic package, reverse it, support the two edges with index and middle fingers of both hands, immerse it in the buffer with the opening downward and gently press the central part of the kit with two thumbs. Thus the gel will fall into the buffer with the side of the well upward. Move the gel to make the well end close to negative electrode of the electrophoresis cell. If bubbles are produced in the sample wells, try to remove them.
2. Sample Loading: Mix Optimized 6× DNA loading buffer and DNA sample at a volume ratio of 1:5. Carefully load prepared Marker and the mixed sample into the wells with pipette successively. The appropriate Maker should be selected as the control well.
3. Electrophoresis: Connect the electrophoresis cell to the power source according to the conventions: Red-Anode and Black-Cathode. Turn on the power source. Note that the DNA sample moves from the negative to the positive (the end near the wells that DNA samples are loaded in is negative). Determine whether to stop electrophoresis according to the migration of the tracking dyes.
4. Observation: Switch off the power source when the electrophoresis finishes. Visualize the band by using a gel documentation system and compare the size of the amplified product with that of Marker.NotesTAE Buffer