| Product Type |
Restriction Enzymes |
| Product Summary |
Restriction Enzymes BsmBⅠ is modified as follows:
- Restriction Enzymes BsmBⅠ belongs to one of the Type IIS type internal cutase, which can identify non-text sequences and cut out the identification sequence. It is often used in Golden Gate assembly.The optimized reaction buffer enables Restriction Enzymes BsmBⅠ to maximize its function. At the same time, the reaction buffer contains reorganized albumin, which can enhance the stability of multiple enzymes.
|
| Product Component |
| Component No. |
Component Name |
Component Size |
Quantity |
| 8052451-1 |
Restriction Enzymes BsmBⅠ (10 units/μL) |
1000 units |
1 bottle |
| 8052451-2 |
10× BsmBⅠ Buffer |
1 mL |
1 bottle |
|
| Restriction Enzyme Site |
- 5’…C G T C T C (N)1↓…3′
- 3’…G C A G A G (N)5↑…5′
|
| Usage |
- Combine the following reaction components on ice in the order indicated:
| Reagent |
Dosage |
| DNAa |
≤ 1 μg |
| Restriction Enzymes BsmBⅠ |
10 units |
| 10× BsmBⅠ Buffer |
5 μL |
| ddH2O |
Up to 50 μL |
- Gently suck or flick the tube wall to mix well (never vortex), then centrifuge instantaneously to collect reaction solution;
- Incubate at 55°C for 15 minutes to 1 hour. It is generally recommended to incubate 5 to 10 units of enzyme per µg plasmid DNA and 10 to 20 units of enzyme per µg genomic DNA for 1 hour. If overnight enzyme digestion reaction is required, please adjust the enzyme amount to 1 unit;
- The enzyme can be heat inactivated at by incubation 80℃ for 20 minutes.
- The combined volume of the enzymes in the reaction mixture should not exceed 1/10 of the total reaction volume，avoiding excessive glycerin in the enzymes caused by the star number activity;
- BsmBI Buffer additives (such as glycerin, salt) are the same as pollutants in the substrate solution (such as salt, EDTA, or ethanol, etc.). The smaller the reaction volume, the stronger the enzyme cutting reaction inhibitory effect;
- Small system recommendation
| DNA |
0.1 μg |
0.5 μg |
| Restriction Enzymes BsmBⅠ |
1 unit |
5 units |
| 10× BsmBⅠ Buffer |
1 μL |
2.5 μL |
| ddH2O |
Up to 10 μL |
Up to 25 μL |
| Total |
10 μLb |
25 μL |
|
| Notes |
a. DNA should be free of phenol, chloroform, ethanol, EDTA, detergents or high concentrations of salts, otherwise enzyme activity will be affected; Methylated DNA inhibits certain restriction enzyme digestion reactions.
b. To avoid evaporation, the incubation time of the 10 μL reaction system should not exceed 1 hour. |
Reviews
There are no reviews yet.