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Restriction Enzymes SgeⅠ
$45.63
- SgeⅠ cleaves DNA targets containing 5-methylcytosine on single-stranded or double-stranded DNA. The SgeⅠ restriction enzyme recognizes the m5CNNG (9/13)^ site and cleaves optimally at 37°C in its unique buffer. To ensure consistent performance, the enzyme storage buffer contains premixed BSA, which enhances enzyme stability and binds to contaminants that may be present in the DNA preparation.
Categories: Molecular Biology, Endonuclease
SKU: 8052481
Product will arrive in 2-3 weeks
Overview
✓ Superior quality and stringent quality control
✓ Identify non-text sequences and cut out the identification sequence
✓ Reorganized albumin premixed in reaction buffer
✓ Maximize enzyme function with optimized reaction buffer
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Product Details
Product Type | Restriction Enzymes | ||||||||||||
Product Summary | Restriction Enzymes SgeⅠ is modified as follows:
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Notes | a. The reaction system can be scaled up or down. The reaction time is not recommended to exceed 1 hour. b. The substrate requires at least 2 units of SgeⅠ recognition sequences to be effectively digested. c. The complete cleavage of methylated DNA depends on the number of recognition sites of SgeⅠ. Additionally, since the DNA products produced by recognition site cleavage can promote non-specific cleavage of SgeⅠ, it is recommended to optimize the amount of SgeⅠ enzyme for the cleavage reaction. |
Storage & Preparation
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Data Images
Citations
Product Documents
Product Datasheet | Download Product Datasheet |
COA | Contact Us |
SDS | Download SDS |
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