| Product Type |
SuperCut Fast Restriction Enzymes |
| Product Summary |
SuperCut Fast Restriction Enzymes are modified as follows:
- SuperCut Fast Restriction Enzymes are a series of genetically engineered restriction enzymes that can accurately complete DNA digestion in 5~15 minutes, suitable for rapid digestion of plasmid DNA, PCR products or genomic DNA.
- SuperCut Fast Restriction Enzymes has the following characteristics: digestion can be completed within 5~15 minutes; share a digestion buffer, which greatly simplifies the digestion reaction system; good enzyme activity redundancy for easy substrate overload or complex template digestion. In addition, dephosphorylation and ligation reagent of Biosci is 100% active in SuperCut Buffer, supporting one-tube reaction and improving the experience of “digestion-ligation-redigestion”.
|
| Product Component |
| Component No. |
Component Name |
Component Size |
Quantity |
| 8052441-1 |
SuperCut Fast Restriction Enzymes XhoⅠ (10 units/μL) |
5000 units |
1 bottle |
| 8052441-2 |
SuperCut 10×Buffer |
1 mL |
3 bottle |
| 8052441-3 |
6× Gel Loading Buffer |
1 mL |
1 bottle |
|
| Restriction Enzyme Site |
- 5’…C↓T C G A G…3′
- 3’…G A G C T↑C…5′
|
| Usage |
- Combine the following reaction components on ice in the order indicated:
|
Plasmid DNA |
PCR product |
Genomic DNA |
| DNAa |
≤ 1 μg |
≤ 0.2 μg |
≤ 5 μg |
| SuperCut Fast restriction Enzymes XhoⅠ |
10 units |
10 units |
30~50 units |
| SuperCut 10×Buffer |
2 μL |
3 μL |
5 μL |
| ddH2O |
Up to 20 μL |
Up to 30 μL |
Up to 50 μL |
- Gently mix or flick the tube wall to mix well (never vortex), then centrifuge instantaneously to collect reaction solution;
- Incubate at 37℃ for 15 minutes (plasmid DNA) or for 15~30 minutes (PCR product) or for 30~60 minutes (genomic DNA);
- Optional: Inactivate the enzyme by heating for 20 minutes at 80℃, and add an appropriate amount of 6× Gel Loading Buffer according to the reaction system to terminate the reaction
|
| Notes |
a. DNA should be free of phenol, chloroform, ethanol, EDTA, detergents or high concentrations of salts, otherwise enzyme activity will be affected; Methylated DNA inhibits certain restriction enzyme digestion reactions. |
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