| Product Type |
WB、ECL |
| Product Summary |
- Superlimit ECL chemiluminescent substrate can be catalysed by horseradish peroxidase labeled on the secondary antibody to produce chemiluminescence reaction and sensitively detect the presence of target protein. The product is developed based on a new generation of enhanced chemiluminescence substrate, and the composition is optimized. This product has low background and good stability, and is ten times more sensitive than ordinary ECL reagent.This product can be used for Western blot of HRP labeled antibody and nucleic acid hybridization detection of HRP labeled probe. It can expose X-ray film, or directly carry out luminometer detection or fluorescent CCD scanning.This product has extremely high sensitivity, can detect (10-100) FG antigen, and can use higher antibody dilution ratio (1:2000-1:10000) to save antibody extremely; It can be operated under natural light without entering the darkroom; It emits rapidly and lasts for a long time.
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| Product Component |
| Component No. |
Component Name |
Component Size |
Quantity |
| 8061011-1 |
A-Luminol Solution |
50 mL |
1 bottle |
| 8061011-2 |
B-Peroxide Buffer |
50 mL |
1 bottle |
|
| Usage |
- This product is commonly used for Western Blot detection of HRP-labeled antibodies. Note the use of HRP-labeled IgG or primary antibody-streptavidin-biotin-HRP.
- Western Blot secondary antibody incubation. After the last wash of the membrane is completed, drain it on filter paper (do not completely dry it). Mix equal volumes of A-Luminol Solution and B-Peroxide Buffe and drop them evenly on the membrane (0.125mL solution/cm2 membrane), incubate at room temperature 1-2 minuets.
- Use filter paper to absorb excess luminescent liquid, place the membrane on a clean and flat plastic wrap, and detect it on a chemiluminescence image.
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