Tissue Single-Cell Preparation Kit
$357.14
Product will arrive in 2-3 weeks
The BioSci Tissue Single-Cell Preparation Kit enables gentle and rapid isolation of cells from fresh solid tissues while maintaining high cell viability, minimal cell debris/clumping, and intact cell surface antigen epitopes. Validated across various human and mouse tissues, the kit provides high quality single-cell suspensions suitable for downstream applications such as flow cytometry, magnetic bead sorting, cell culture, and single-cell sequencing.
The kit can be used independantly for mannul tissue dissociation and single cell preparation, however, it is recommended to use with BioSci’s Tissue Dissociator and Tissue Dissociation Spin Tube.
The kit is 24 RCTs / kit.
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Product Details
The Tissue Single-cell Preparation Kit contains a mixture of digestive enzymes with different mechanisms of action. It rapidly dissociates tissue into a single-cell suspension while maintaining cell viability by disrupting interstitial components and intercellular connections through mechanical grinding/shearing and enzymatic digestion.
24 RCTs per kitl. Component:
Lyophilized Enzyme Reagent (Enzyme A) 2 mg/vial 6 tubes
Lyophilized Enzyme Reagent (Enzyme B) 20 mg/vial 6 tubes
Lyophilized Enzyme Reagent (Enzyme C) 1 mg/vial 6 tubes
Tissue Single-Cell Preparation Buffer 20 mL/bottle 6 bottles
Range of Tissue Weight Recommended Volume of Tissue Single-Cell Preparation Solution
10 mg < Tissue Weight ≤ 50 mg 0.5 mL~1 mL
50 mg < Tissue Weight ≤ 100 mg 1 mL~2 mL
100 mg < Tissue Weight ≤ 500 mg 2 mL~3 mL
500 mg < Tissue Weight ≤ 1 g 3 mL~5 mL
Tissue Weight > 1 g 5 mL~7 mL
Storage & Preparation
Transport at 2-8 ℃.
The lyophilized enzyme reagent should be stored at -25 ℃ to -15 ℃, the tissue single-cell preparation buffer should be stored at 2 ℃ to 8 ℃.
After aliquoting the ready-to-use mix solution, store frozen at -25 ℃ to -15 ℃. Avoid repeated freeze-thaw cycles.
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