XhoⅠ Elisa Kit
$443.66
- This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for XhoⅠ has been precoated onto a microplate. Standards and samples are pipetted into the wells and any XhoⅠ present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for XhoⅠ is added to the wells. Following a wash to remove any unbound antibodyenzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of XhoⅠ bound in the initial step. The color development is stopped and the intensity of the color is measured. And the residual degree of XhoⅠ in the sample is measured.
Product will arrive in 2-3 weeks
Overview
- Application: ELISA
- Detection range: 0.125~4 ng/mL
- Quantification limit: 0.125 ng/mL
- Detection limit: 0.021 ng/mL
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Product Details
| Product Type | ELISA | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| Notes | a. Bring the required reagents to room temperature (25±3℃) before use. Determine the number of wells to be used for the assay, remove the required number of microplate strips, reseal the remaining strips, and store at 2~8℃. b. 1× Wash Buffer Preparation: Based on the volume of wash buffer required for this experiment, measure an appropriate amount of 20× wash buffer and dilute with deionized water at a 1:20 volume ratio, mix well, and set aside for use. c. Do not let the plate wells dry excessively for a long time. Improper washing may lead to a higher CV value between replicate wells, and signal bias either high or low. |
Storage & Preparation
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Data Images
Note: BsaⅠ standard curve ranging from 0.125 to 4 ng/mL.
Citations
Product Documents
| Product Datasheet | Download Product Datasheet |
| COA | Contact Us |
| SDS | Download SDS |
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