RPMI-1640 Medium

with

• L-glutamine
• phenol red
• sodium bicarbonate

without

• sodium pyruvate
• HEPES
• antibiotics

• RPMI-1640 requires additional culture additives such as fetal bovine serum. 
• It uses a sodium bicarbonate buffer system and therefore requires a 5% ~ 10% CO₂ environment to maintain physiological pH.
• It has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and hybridomas.